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Crucial elements that maintain the interactions between the regulatory TnaC peptide and the ribosome exit tunnel responsible for Trp inhibition of ribosome function

机译：维持调节性TnaC肽和核糖体出口通道之间相互作用的关键要素，该通道负责Trp抑制核糖体功能

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Translation of the TnaC nascent peptide inhibits ribosomal activity in the presence of l-tryptophan, inducing expression of the tnaCAB operon in Escherichia coli. Using chemical methylation, this work reveals how interactions between TnaC and the ribosome are affected by mutations in both molecules. The presence of the TnaC-tRNAPro peptidyl-tRNA within the ribosome protects the 23S rRNA nucleotide U2609 against chemical methylation. Such protection was not observed in mutant ribosomes containing changes in 23S rRNA nucleotides of the A748–A752 region. Nucleotides A752 and U2609 establish a base-pair interaction. Most replacements of either A752 or U2609 affected Trp induction of a TnaC-regulated LacZ reporter. However, the single change A752G, or the dual replacements A752G and U2609C, maintained Trp induction. Replacements at the conserved TnaC residues W12 and D16 also abolished the protection of U2609 by TnaC-tRNAPro against chemical methylation. These data indicate that the TnaC nascent peptide in the ribosome exit tunnel interacts with the U2609 nucleotide when the ribosome is Trp responsive. This interaction is affected by mutational changes in exit tunnel nucleotides of 23S rRNA, as well as in conserved TnaC residues, suggesting that they affect the structure of the exit tunnel and/or the nascent peptide configuration in the tunnel.
机译：TnaC新生肽的翻译在l-色氨酸存在下抑制了核糖体活性，从而诱导了tnaCAB操纵子在大肠杆菌中的表达。使用化学甲基化，这项工作揭示了两个分子的突变如何影响TnaC和核糖体之间的相互作用。 TnaC-tRNAPro肽基-tRNA在核糖体中的存在可保护23S rRNA核苷酸U2609免受化学甲基化的影响。突变的核糖体在A748–A752区域的23S rRNA核苷酸中有变化，但未观察到这种保护。核苷酸A752和U2609建立碱基对相互作用。 A752或U2609的大多数替代品都会影响TnaC调控的LacZ报告基因的Trp诱导。但是，单个更改A752G或双重替换A752G和U2609C保持了Trp诱导。保守的TnaC残基W12和D16的置换也取消了TnaC-tRNAPro对U2609的化学甲基化保护。这些数据表明，当核糖体对Trp响应时，核糖体出口通道中的TnaC新生肽与U2609核苷酸相互作用。这种相互作用受到23S rRNA出口隧道核苷酸以及保守的TnaC残基突变的影响，表明它们影响出口隧道的结构和/或隧道中新生的肽构型。

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